The Basic Principles Of attune flow cytometer
The Basic Principles Of attune flow cytometer
Blog Article
Facts-enriched characterization of cell populations. Morphological information from images can incorporate into the richness of flow cytometry details. As an example, the determine displays an otherwise regular apoptosis assay utilizing Annexin V and PI, incorporating cell imaging to characterize cells in Each and every population to reveal morphologically unique features. These insights could not are already acquired from multiplex staining alone.
As samples are acquired to the Attune CytPix Flow Cytometer, the higher-speed brightfield digital camera captures and stores illustrations or photos of detected gatherings in a price approximately six,000 photographs per next, based on the flow rate and image dimensions. For larger overall flexibility, Attune Cytometric Computer software helps you to regulate the graphic capture frequency as required and select a selected gate for picture seize, if demanded.
Incorporate acoustic focusing technologies with high-parameter Investigation for adaptable spectral unmixing and conventional workflows.
Discover new insights into your experimental data by visualizing populations utilizing mixed fluorescence and morphology details. Our software interface wizards empower downsampling, concatenation (merged information) and dimensionality tools which might be crafted into your program for an intuitive, streamlined information Examination experience.
"An image is just not rather worth one thousand dots, nonetheless it truly really helps to see images. It is great to view pictures that correlate with staining. For example, AnnexinV-favourable cells which have been morphologically distinct than healthier cells, or CD14+ monocytes that happen to be larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Moreover, it truly is illuminating to check out the number of doublets slip by classic singlet gates, and terrific to verify the standing of unforeseen double-favourable events in my Assessment."
Pair your Attune flow cytometer having an autosampler for elevated workflow effectiveness, and incorporate robotic integration for optimum throughput.
Our researchers will structure panels in your case as often times you would like—totally free, no product or service buy required.
The Attune NxT cytometer is the trusted workhorse instrument your lab can depend upon for reliable and trusted overall performance. The Attune CytPix product includes a large-pace brightfield digital camera and AI-driven graphic analysis software program, with current updates together with machine learning resources and an intuitive person interface, enabling enhanced data precision, accuracy, and a lot quicker insights into sample biology.
Potentially most of all, these gatherings consist of cells of Obviously different phenotypes which may lead to incorrect conclusions relating to double constructive occasions (specifically in unusual populations).
AI-driven automated picture Investigation Along with the Attune Cytometric Software interprets celebration options into distinctive morphology parameters that may be combined with normal fluorescence and scatter parameters to expose very reproducible inhabitants improvements and exclusive subsets.
Application Observe: Automated picture analysis reduces person-to-consumer variability in flow cytometry gating tactics
Constructed-in dimensionality reduction equipment amplified resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to substantiate anticipated populace resolution in lysed complete human blood. Consumer can see events from Each individual cluster for Visible confirmation of mobile phenotypes.
Flexible payment prepare from Agilent Fiscal Methods enable you to obtain the essential instrumentation you require for speedy job, or to expand your lab.
At the heart of every Attune Flow Cytometer lies our revolutionary acoustic focusing technologies, which utilizes seem waves to meticulously align cells inside the flow mobile. This impressive technique surpasses regular hydrodynamic procedures, leading read more to a lot quicker processing speeds, Extraordinary details top quality, and improved sample throughput.
The Attune Xenith cytometer is really a substantial-parameter spectral analyzer, giving the two spectral unmixing and traditional flow abilities for comprehensive and versatile mobile analysis.
The Attune CytPix Flow Cytometer enables customers to visualize samples during acquisition, validate the accuracy of gating procedures, and support final results with responsible documentation for publication functions.
What gave the impression to be a homogeneous population is really far more varied—an insight that needs to be deemed when interpreting final results.